WebSep 4, 2003 · The sulfhydryl-reactive reagent, BMCC (Pierce Endogen) was used to probe reaction with Cys substitutions at positions in the N-terminal region of BtuB (residues 1–17 and 25–31) and to detect changes in labeling in the presence of BtuB transport substrates. BMCC contains a maleimide moiety joined to biotin through a cyclohexyl-butyl linker. WebApplications. Anti-AGE (Advanced Glycation End-products) Antibody detects level of AGE (Advanced Glycation End-products) & has been published & validated for use in ELISA & WB. Western blot: >=1:2,000 on AGE-modified proteins. ELISA: >= 1:4,000 against Glycoaldehyde-modified BSA.
Characterization for Binding Complex Formation with Site ... - Hindawi
WebFeb 18, 2013 · Biotin-BMCC is a maliemide conjugated biotin molecule that has a high affinity for free thiol groups of cysteines (Figure 1). While performing step 4.2, prepare 0.5 ml of Biotin-BMCC Buffer (Table 1) per sample, at … WebNov 11, 2014 · At the same time, the immunoprecipitates were treated with 50 mM N-ethylmaleimide to block free sulfhydryl groups, incubated with 1 M hydroxylamine to hydrolyze any Cys-palmitate thioester bonds, and then treated with 1 μm EZ-Link Biotin-BMCC in 50 mM Tris (pH 7.0) to label the newly exposed free sulfhydryl groups resulting … diagnostic test in mapeh 5 with tos
Validation of ABE method with substrate SNAP25. A
WebThe optimal amount of BMCC-Biotin to use for each reaction depends on a number of factors. By regulating the reagent-to-target molar ratio in the reaction, the extent of … WebOct 26, 2010 · A variety of different thiol-specific compounds containing biotin, such as biotin-BMCC, biotin-PEO-maleimide, biotin-PEO-iodoacetamide or biotin-HPDP, have been applied to label the free sulfhydryl groups liberated after cleaving palmitate from palmitoylation sites . WebThe conjugate between biotin–BMCC (Fig. 1A) and GSH was analyzed with an ABI 3000 electrospray mass spectrometer (Applied Biosystems, Foster City, CA) in positive ion mode. A 20 μM sample of BMCC–GSH conjugate was infused into the mass spectrometer, using a Harvard syringe pump, at a flow rate of 20 μl/min. diagnostic testing urethritis