Phospho-sting-s366
Web靶标信息. STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF …
Phospho-sting-s366
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WebPhospho-STING (Ser366) two-plate assay protocol. The two-plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before adding HTRF Phospho-STING (Ser366) detection reagents. This protocol enables the cells' viability and confluence to ... WebFeb 7, 2024 · TBK-independent phosphorylation of STING(S366) and its adaptor molecule TICAM2 by phospho-AKT(T308S473) augmented downstream IRF3-dependent IDO1 transcription, which was also dependent on β-catenin.
WebWe also generated a rabbit polyclonal antibody specific for S366-phosphorylated hSTING (p-S366); this antibody detected phosphorylated hSTING in HT-DNA transfected cells but not … WebOct 24, 2013 · We conclude that phosphorylation of S366 (S365 in murine STING) occurs after autophagy and may negatively regulate STING activity to prevent sustained type I IFN production following activation by cytosolic dsDNA. Download : Download high-res image (515KB) Download : Download full-size image Figure S2.
WebApr 6, 2024 · Binding of TBK1 to this motif promotes the phosphorylation of S366 in the STING tail 19, which subsequently recruits and promotes the phosphorylation of the transcription factor interferon... WebMay 1, 2024 · (A) Heatmap shows correlation of phospho-STING (Ser_366) protein levels with cGAS and other immune-related proteins in the MD Anderson PROSPECT cohort, …
WebPhosphorylated S366 is a docking site for IRF3 and is essential for the subsequent induction of Interferons (IFNs) ( 7, 8 ). Our previous work demonstrated that IRF3 binds to phosphorylated STING in the late endosomal compartment, where TBK1 phosphorylates and activates IRF3 ( 9 ). Additional proteins regulate the trafficking of STING.
Phospho-STING (Ser366) (D7C3S) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser366 of human STING protein. Background sushi for pregnant womenWebOct 1, 2024 · Phosphorylation of S358 enables TBK1 to bind STING. TBK1 then trans-phosphorylates S366 of neighboring STING complexes, allowing the recruitment ... [138]. Paradoxically, introduction of the phospho-mimic S366D also strongly reduces IRF3 dimerization and IFN I production [163, 164]. This may be due to clearance of … sushi for toddlersWebHTRF phospho-STING (S366) 64STGPEG HTRF total-STING 64NTGPEG HTRF phospho-TBK1 (S172) 64TBKPEG HTRF total-TBK1 64NTBPEG HTRF phospho-IRF3 (S386) 6FRF3PEG HTRF human IFNb 62HIFNBPEG Compounds References 2’3’ cGAMP C161-Biolog c-diAMP C088-Biolog c-diGMP C057-Biolog DMXAA D5817 –Sigma-Aldrich. sushi for reelWebPhospho-STING (Ser366) (E9A9K) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / Purification Monoclonal antibody is produced … sushi for people who dont eat seafoodWebSTING (human) S366-p. 19781 : Phospho-STING (Ser366) (D7C3S) Rabbit mAb sushi for pregnancyWebWestern blot - Phospho-STING-S365 Rabbit pAb (AP1199) Western blot analysis of extracts of THP-1 cells, using Phospho-STING-S365 antibody (AP1199) at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. sushi for robotsWebJan 29, 2015 · STING S366 phosphorylation after HT-DNA transfection was detected with the p-S366 antibody (fig. S8E). ( E ) S366 phosphorylation of endogenous STING in THP-1 … sushi for you huizen