Stat6 reporter assay
WebJan 31, 2024 · The STAT6 inhibitor AS1517499 was used to specifically inhibit p-STAT6. The effect of p-STAT6 inhibition on Ph+ CML and Ph+ ALL cells was identified by CCK-8 and FCM assay. Dual luciferase reporter and ChIP assay were performed to confirm the direct binding between STAT6 and c-Myc. WebDec 10, 2024 · Beta actin was used as internal control. (F) Reporter assays of STAT6 promoter. HEK293 cells co-transfected STAT6 promoter-driven luciferase reporter with different dosage RTA (0, 1, 5, 10μg) were subjected to reporter assay. Relative firefly luciferase unit (RLU) normalization with Renilla activity was analyzed. Data is presented …
Stat6 reporter assay
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WebReporter gene assays demonstrated that, in an IL-4– and STAT6-dependent manner, STAT6 inhibited activation of the PPARα-responsive luciferase reporter (Fig. 2A). This transrepression of STAT6 required PPARα, because transfection of STAT6 alone had negligible effect ( Fig. S2 ). WebJan 31, 2024 · Dual luciferase reporter and ChIP assay were performed to confirm the direct binding between STAT6 and c-Myc. The impact of STAT6 inhibition on tumor progression was detected in Ph+ CML and Ph+ ALL mouse models. Results Our results demonstrated that P210 induced CML-like disease, and P190 caused the more malignant ALL-like …
WebDec 15, 1998 · We report here that Stat6 interacts with three binding sites in the human IL-4 promoter by electrophoretic mobility shift assays. These sites overlap the P1, P2, and P4 … WebDescription. The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection …
WebMay 15, 2024 · Dual-luciferase reporter assay. Targetscan was used to predict the up-stream regulatory gene of STAT6, and miR-135a-3p was proved to regulate STAT6. ... The viability of RA FLSs transfected with si-STAT6 was assessed by CCK-8 assay at indicated times; (B) The proliferation of RA FLSs transfected with si-STAT6 was evaluated by EdU … WebMay 30, 2024 · Approximately 40,000 HEK293-hSTAT6/5×STAT6-luc cells resuspended in 50 μl assay medium were seeded and incubated at 37 °C with 5 % CO2 for 6 h. After …
WebDescription STAT6-Luc Reporter Cell Line-Ba/F3 stably expresses luciferase reporter gene under the control of the STAT6 responsive promoter. This cell line is an ideal cellular model for monitoring STAT6 signaling pathway activities. Host Cell Ba/F3 Shipping Dry ice Storage Liquid nitrogen Customer Reviews
WebJul 18, 2024 · Reporter assays showed increased IL-4 induced transactivation activity of STAT6 MUT at the PARP14 promotor, suggesting a self-reinforcing regulatory circuit. Knock-down of PARP14 or... of the origin of speciesWebThe results from the reporter assays strongly suggested that PU.1 facilitates Stat6‐mediated transcription by directly binding to its core binding sequence in the DNA within the Stat6 response element on the Igϵ promoter. However, it was necessary to investigate whether the point mutation affected the DNA‐binding of Stat6. of the outer skin crossword clueWebSTAT Reporter Cell Line - Tool for Screening of Drugs Targeting JAK/STAT Pathway Signal transducer and activator of transcription (STAT) protein is a family of transcription factors … of the original contract hume summaryWebJun 6, 2024 · Additionally luciferase reporter assay showed that the inhibited activity of SLC7A11 promoter by P53 were attenuated by STAT6 overexpression or CBP knockdown, which was confirmed by ChIP assay ... my friend wears diapersWebOct 24, 2024 · When transfecting STAT6-expressing plasmid pLS16 (P hCMV-STAT6-pA) and reporter plasmid pLS12 ... The histamine release assay was performed in an incubator at 37 °C in a humidified 5% CO2-containing atmosphere for 30 min. Thereafter, the cells were centrifuged for 10 min at 700 ... my friend who lived in new yorkWebA bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and … of the ox family crossword clueWebCyclooxygenase-2 (COX-2) is frequently overexpressed in human cancers and contributes to the malignant phenotype. Our data indicate unphosphorylated signal transducers and activators of transcription of the palm of one\u0027s hand crossword clue