T4 dna ligase hc

Author: hcmu

August undefined, 2024

WebApr 14, 2024 · The products were ligated with T4 DNA ligase (TaKaRa, Dalian, Liaoning, China) at 16 °C o/n. The recombinant plasmids were confirmed by Sanger sequencing (Sangon Biotech, Shanghai, China) and co-transfected with pRL-TK plasmid (Renilla luciferase). After 24 h incubation, luciferase activities were measured with Dual … WebThermostable DNA ligase which efficiently seals nicks and discriminates against mismatch ligation. Add to Cart: 100µmol dNTP Solution Mix Add to Cart: N2050L: 100 µmol: ... T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 ...

Properties of DNA and RNA Ligases NEB

WebT4 DNA Ligase Part Number: L603 -HC L Lot Number: * For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section below . 100 Cummings Center, Beverly, MA 01915 • (888) 927-7072 • (978) 927-7172 • www.enzymatics.com Limitations of Use WebJul 13, 2024 · Commercial enzymes (Phusion High-Fidelity DNA Polymerase, BamHI, XhoI, T4 polynucleotide kinase (3’ phosphatase minus) and T4 DNA ligase were purchased from New England BioLabs, Inc. (Bethesda, MD, USA). ... Bands (HT = holotoxin; HC = heavy chain; LC = light chain) ... lainer bau landshut

T4 DNA Ligase Ligase DNA Ligation - Promega

WebAug 28, 2010 · PCR amplification-purified cDNA was cloned into the pGEM-T easy vector (Promega) by T4 DNA ligase and amplified in Escherichia coli, JM109. Four positive colonies were selected and assessed using EcoRIdigestion of mini-prepared DNA. Putative MAGE-1, SSX-1 and CTp11 cDNA samples were sequenced with T7 sequencing primers … WebEZ L6030-HC-L: T4 DNA Ligase (Rapid) 240.000 units (600.000 U/ml) EZ L6030-LC-L: T4 DNA Ligase 150000 units (120,000 U/ml) Westburg is an independent distributor providing high quality, innovative products to Life Sciences laboratories. We offer you a combination of products, technical know-how and excellent service. WebIt is the highest concentration (50 U/μL) T4 enzyme… Unlock the full potential of your diagnostic assays with Glycerol-Free T4 DNA Ligase (HC). Liked by Taylor Sealy laine lang yarns soldes

T4 DNA Ligase NEB

WebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins … WebT4 DNA Ligase (Rapid) Part Number -HC L Concentration 600,000 U/mL Unit Size 240,000 U Storage Temperature -25⁰C to -15⁰C . Product Specifications L6030-HC-L Rev F Limitations of Use This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for administration to humans or jema galanza uaapWebThe unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme. Applications Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA Source Purified from E. coli lambda lysogen NM989 Performance and quality testing jema galanza trending pic

"WebHi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability compared to T4 DNA Ligase Capable of joining blunt end and cohesive end termini, as … " - T4 dna ligase hc

T4 dna ligase hc

13-Fragment Golden Gate Assembly Protocol using SapI - NEB

http://www.enzymatics.com/pdf2/L6030-HC-L.pdf WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA …

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WebAll Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert. ... T4 DNA Ligase (HC) M179A: 1 × 500u: 5X Flexi® Digest Buffer. R186A: 1 × 1ml: 2X Flexi® Ligase Buffer. R187A: 1 × 500μl: Nuclease-Free Water. P119A: 2 × 1 ... WebThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The …

WebJun 5, 2024 · For assemblies involving 10 fragments and less, the standard amounts (500 units T4 DNA Ligase, 15 units BsaI-HFv2) are sufficient. Note the reaction volume of 25 µl is used to allow sufficient volume for precloned insert additions, if needed. Assembly Reactions. Set up 25 µl assembly reactions as follows: WebThe mixture of 10uM Forward primer with 5’ end, 10X T4 DNA ligase buffer, T4 PNK (10 units) and Nuclease-free water 1) incubated at 37°C for 1 hour, and 2) incubated at 65°C for 20 minutes to ...

WebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate … WebT4 DNA Ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. 10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl 2, …

Web1,000 units of T4 DNA Ligase (NEB #M0202) or Hi-T4 DNA Ligase (NEB #M2622) in (1X) T4 DNA Ligase Buffer were cycled in the presence of DNA at 42°C (A) or 50°C (B) for 5 minutes followed by 16°C for 5 minutes in the presence of 1 μg pBR322. Following the indicated number of cycles (0, 15, 30, 45 or 60), ligase activity was tested on a ...

WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The … lainerhof salzburgWebForensic DNA Analysis (CE) Forensic DNA Analysis (MPS) Lab Equipment & Supplies. Biochemicals & Labware; CE Instruments; Plate Readers, Fluorometers & … jema gard 2022WebTth DNA Ligase For ligase chain reaction and ligase detection reaction T4 DNA Ligase MBG For joining both blunt-ended and cohesive-ended restriction fragments of DNA … je mag altijd bij me komenWebApr 6, 2024 · T4 ligase buffer was classically found to be superior to ATP-supplemented NEBuffer 3 for T4 ligase Golden Gate assembly. Engler Barrick Lab found NEBuffer 3.1 with 42° digests to be best for T7 ligase BsmBI reactions, better than CutSmart buffer, ligase buffer, or any with 37° digests; to be expected as BsmBI prefers high salt/temp. jemagazine caWebJun 10, 2013 · For the T4 DNA ligase variants, each 10 µl activity assay contained 150 ng of this DNA substrate (∼40 nM ligatable DNA ends) and a ligase (2 µM), in 1× New England Biolabs T4 DNA Ligase Reaction Buffer (50 mM Tris-HCl, 10 mM MgCl 2, 10 mM dithiothreitol, 1 mM ATP, pH 7.5). Reactions were incubated for 10 min at 16°C, before … jemagazine çaWebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 … jemagazine.ca coolWebApr 12, 2024 · The C terminus of KsgA has a similar structure to H2TH domains of MutM and Nei. Residues 129-229 of MutM (full length 269 amino acids) and 126-189 of Nei (full length 263 amino acids) constitute the H2TH domain that binds to DNA in order to repair damaged bases [15, 43, 44].A previous study reported that residues 214-273 of KsgA … jemagazine/avis